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Q. No.In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
| 1. | recognition sequence | 2. | cloning site |
| 3. | restriction site | 4. | ori site |
Subtopic: Principles of Biotechnology |
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Level 1: 80%+
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
| 1. | D and E only | 2. | B and C only |
| 3. | A and E only | 4. | A and B only |
Subtopic: Principles of Biotechnology |
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA move toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Separation and Isolation of DNA fragments |
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Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
| 1. | Hind II, Hind II | 2. | Hind II, Alu I |
| 3. | Hind II, EcoR I | 4. | Hind II, Bam HI |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
64%
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
87%
Level 1: 80%+
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Which of the following are correct about EcoRI?
Choose the correct answer from the options given below:
| A. | Cut the DNA with blunt end |
| B. | Cut the DNA with sticky end |
| C. | Recognises a specific palindromic sequence. |
| D. | Cut the DNA between the base G and A where it encounters the DNA sequence 'GAATTC' |
| E. | Exonuclease |
| 1. | B,C,E only | 2. | A,D,E only |
| 3. | A,C,D only | 4. | B,C,D only |
Subtopic: Restriction Enzymes - Main Enzymes |
81%
Level 1: 80%+
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
89%
Level 1: 80%+
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 2: 60%+
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
| 1. | 6 bp | 2. | 4 bp |
| 3. | 10 bp | 4. | 8 bp |
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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Level 1: 80%+
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The " Ti plasmid " of Agrobacterium tumefaciens stands for
| 1. | Tumor independent plasmid |
| 2. | Tumor inducing plasmid |
| 3. | Temperature independent plasmid |
| 4. | Tumour inhibiting plasmid |
Subtopic: Tools: Vectors: I |
83%
Level 1: 80%+
NEET - 2024
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