| List-I | List-II | ||
| A. | Genetically engineered Human Insulin | I. | Gene therapy |
| B. | GM Cotton | II. | E.Coli |
| C. | ADA Deficiency | III. | Antigen-antibody interaction |
| D. | ELISA | IV. | Bacillus thuringiensis |
| 1. | creating pores in the midgut |
| 2. | damaging the respiratory system |
| 3. | degenerating the nervous system |
| 4. | altering the pH of body fluids |
| A. | The inactive toxin converted into active form due to alkaline pH of gut of insect |
| B. | Bacillus thuringiensis produce crystals with toxic insecticidal proteins. |
| C. | The alkaline pH solubilises the crystals. |
| D. | The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. |
| E. | The toxin proteins exist as inactive protoxins in bacteria. |
| Statement I: | RNA interference takes place in all eukaryotic organisms as method of cellular defense. |
| Statement II: | RNAi involves the silencing of a specific mRNA due to a complementary single stranded RNA molecule that binds and prevents translation of mRNA |
| 1. | Bt toxin requires acidic pH for activation |
| 2. | Bacillus has protective mechanism against it |
| 3. | In Bacillus, it is present in an inactive state and once insects ingests it, it is converted to active form |
| 4. | The protoxin requires protein conjugate for its activation |
| Statement I: | Bt toxins are insect group specific and coded by a gene cry IAc. |
| Statement II: | Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect, the inactive protoxin gets converted into active form due to acidic pH of the insect gut. |
| List-I | List-II | ||
| A. | \(\alpha-1\) antitrypsin | I. | Cotton bollworm |
| B. | Cry IAb | II. | ADA deficiency |
| C. | Cry IAc | III. | Emphysema |
| D. | Enzyme replacement therapy | IV. | Corn borer |
| List-I | List-II | ||
| (A) | Gene therapy | (I) | Separation of DNA fragments |
| (B) | RNA interference | (II) | Diagnostic test for AIDS |
| (C) | ELISA | (III) | Cellular defense |
| (D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
| Options: | (A) | (B) | (C) | (D) |
| 1. | IV | I | II | III |
| 2. | IV | II | III | I |
| 3. | IV | III | II | I |
| 4. | IV | III | I | II |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
| Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
| Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |